Optimization of some environmental and nutritional conditions using microtiter plate for Pseudomonas aeruginosa biofilm formation

One of the most important virulence factors in Pseudomonas aeruginosa is biofilm formation, as it works a barrier for entering antibiotics into bacterial cell. Different environmental and nutritional conditions were used to optimize formation using microtitre plate assay by P. aeruginosa. The low nutrient level medium represented tryptic soy broth (TSB) was better than high with Luria Broth (LB). optimized condition production at room temperature (25 °C) host (37 °C). Moreover, staining 0.1% crystal violet reading wavelength 360 are considered essential increasing productivity Finally, we highly recommended these assays assess